FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. The invention is a liquid nutrient medium for the deep cultivation of a vaccine strain of the plague microbe Y. pestis EV, containing the following ingredients: acid hydrolysate of corn syrup 88.0 ml, sodium chloride 5.0 g, sodium phosphate di-substituted 12-water 4.0 g, Mohr’s salt 0.04 g, sodium sulphate 0.35 g, distilled water up to 1 l, stimulating additives of sodium sulfite and Mohr’s salt.
EFFECT: invention provides a high percentage of living microbial cells in the biomass after 20 h.
1 cl
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Authors
Dates
2021-10-15—Published
2021-01-21—Filed