FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biochemistry and represents a new bifunctional PSH protein containing human peroxiredoxin Prx6 and manganese superoxide dismutase MnSOD possessing the antioxidant activity of superoxide dismutase and peroxidase. What is also described is a chimeric nucleic acid coding the presented protein. A method for preparing the presented protein by culturing cells of the strain E.coli BL21(PSH) transformed by constructed recombinant expression vector based on pET22b(+) plasmid is disclosed.
EFFECT: invention enables producing high-yield protein PSH possessing the high antioxidant activity.
4 cl, 6 dwg, 3 tbl, 7 ex
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