FIELD: biotechnologies.
SUBSTANCE: invention represents plasmid pET40CmAP/MBL-T determining a synthesis of hybrid bifunctional polypeptide CmAP/MBL-T with properties of high-activity alkaline phosphatase of sea bacterium Cobetia marina (CmAP) and mannan-binding lectin of C-type of far-eastern trepang Apostichopus japonicus (MBL-T). The plasmid includes NcoI/SalI - a fragment of plasmid pET-40b(+) (Novagen) and a DNA fragment with size of 2034 pairs of bases, which is a chimeric gene coding structural genes of alkaline phosphatase CmAP and mannan-binding lectin of C-type MBL-T, which are connected to each other with a polynucleotide coding the amino acid sequence of linker (G)4S(G)4S(G)4SEL. The invention describes recombinant strain E.coli Rosetta(DE3)/pET40CmAP/MBL-T transformed by the above plasmid - a producer of hybrid bifunctional protein CmAP/MBL-T. The invention also proposes a method for obtaining hybrid bifunctional protein CmAP/MBL-T using the proposed strain.
EFFECT: invention allows obtaining the specified highly purified preparation of a hybrid protein with properties of recombinant high-activity alkaline phosphatase CmAP and highly specific mannan-binding lectin of C-type MBL-T with regard to carbohydrate components of glycoproteins of cancer cells of uterine cervix and can be used for a high-sensitivity lectin-immunoferment method of early differential diagnostics of cervical cancer.
3 cl, 2 dwg, 3 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pET40CmAP/CGL ENCODING HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/CGL WITH PROPERTIES OF HIGHLY ACTIVE ALKALINE PHOSPHATASE CmAP AND GALACTOSE SPECIFIC LECTIN CGL, RECOMBINANT STRAIN OF E coli Rosetta (DE3)/pET40CmAP/CGL - PRODUCER OF HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/CGL AND METHOD OF ITS PREPARATION |
2014 |
- Balabanova Larisa Anatol'Evna
- Golotin Vasilij Aleksandrovich
- Koval'Chuk Svetlana Nikolaevna
- Chernikov Oleg Viktorovich
- Chikalovets Irina Vladimirovna
- Rasskazov Valerij Aleksandrovich
|
RU2557306C1 |
| RECOMBINANT PLASMID pET40CmAP/ MPF DNA, ENCODING BIFUNCTIONAL HYBRID CmAP/OmpF POLYPEPTIDE WITH PROPERTIES OF HIGHLY-ACTIVE ALKALINE PHOSPHATASE CmAP AND PORE-FORMING MEMBRANE OmpF PROTEIN, AND RECOMBINANT STRAIN OF E. COLI ROSETTA (DE3)/pET40CmAP/OmpF - PRODUCER OF BIFUNCTIONAL HYBRID CmAP/OmpF POLYPEPTIDE |
2016 |
- Balabanova Larisa Anatolevna
- Golotin Vasilij Aleksandrovich
- Bujnovskaya Nina Sergeevna
- Portnyagina Olga Yurevna
- Novikova Olga Daniilovna
- Rasskazov Valerij Aleksandrovich
|
RU2634871C1 |
| ALKALINE PHOSPHATASE CmAP SYNTHESIS-DETERMINING 40Ph PLASMID, E. coli rosetta(DE3)/40Ph STRAIN - PRODUCER OF CHIMERIC PROTEIN, CONTAINING AMINO ACID SEQUENCE OF RECOMBINANT ALKALINE PHOSPHATASE CmAP, AND PRODUCTION METHOD THEREOF |
2010 |
- Balabanova Larisa Anatol'Evna
- Rasskazov Valerij Aleksandrovich
|
RU2447151C1 |
| RECOMBINANT PLASMID DNA PCSBRZ4M ENCODING A POLYPEPTIDE WITH BRAZZEIN SWEET PROTEIN PROPERTIES AND A RECOMBINANT E. COLI STRAIN PRODUCING A POLYPEPTIDE WITH BRAZZEIN SWEET PROTEIN PROPERTIES |
2022 |
- Gerasimov Andrei Sergeevich
- Misterova Anna-Anastasiia Viktorovna
- Rogozhkin Sergei Olegovich
- Sazanova Evgeniia Vitalevna
- Chicherin Vladimir Aleksandrovich
|
RU2799530C1 |
| PLASMID 40NaGal, DETERMINING SYNTHESIS OF α-N-ACETYLHALACTOSAMINIDASE OF α-AlNaGal, STRAIN E.coli Rosetta(DE3)/40NaGal - PRODUCER OF CHIMERIC PROTEIN, WHICH INCLUDES AMINOACID SEQUENCE OF RECOMBINANT α-N-ACETYLHALACTOSAMINIDASE OF α-AlNaGal, AND METHOD OF ITS OBTAINING |
2013 |
- Balabanova Larisa Anatol'Evna
- Golotin Vasilij Aleksandrovich
- Bakunina Irina Jur'Evna
- Rasskazov Valerij Aleksandrovich
|
RU2525682C1 |
| RECOMBINANT pHisTevTSIB0821 PLASMID; Escherichia coli Rosetta(DE3)/pHisTevTSIB0821 STRAIN TRANSFORMED BY ABOVE SAID PLASMID, AND METHOD FOR OBTAINING RECOMBINANT TSIB_0821 PROLIDASE |
2012 |
- Popov Vladimir Olegovich
- Fateeva Tat'Jana Vladimirovna
- Judkina Ol'Ga Vladimirovna
- Korzhenevskij Dmitrij Andreevich
- Rakitina Tat'Jana Vladimirovna
- Cherkashina Anna Sergeevna
- Slutskaja Ehl'Vira Semenovna
- Lipkin Aleksej Valer'Evich
|
RU2509154C1 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli |
2013 |
- Petrovskaja Lada Evgen'Evna
- Shingarova Ljudmila Nikolaevna
- Gapizov Sultan Shakhbanovich
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Jakimov Sergej Aleksandrovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2527171C1 |
| RECOMBINANT DNA ENCODING FUNCTIONALLY ACTIVE HYBRID PROTEIN OF D-AMINO ACID OXYDASE WITH CHITIN-COUPLING DOMAIN (DAOcbd), pVR1 RECOMBINANT PLASMID PROVIDING SYNTHESIS THEREOF IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C 41 (DE3)/pVR1 AS PRODUCER OF DAOcbd |
2006 |
- Redo Vadim Aleksandrovich
- Ehl'Darov Mikhail Anatol'Evich
- Khatuntseva Svetlana Anatol'Evna
- Zhgun Aleksandr Aleksandrovich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2310688C1 |
| RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE |
2015 |
- Petrovskaya Lada Evgenevna
- Shingarova Lyudmila Nikolaevna
- Kryukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Zlobinov Aleksandr Vladimirovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2606014C1 |
| PLASMID 40Gal DETERMINING SYNTHESIS OF α-GALACTOSIDASE α-PsGal, STRAIN Ecoli Rosetta(DE3)/40Gal - PRODUCER OF CHIMERIC PROTEIN CONTAINING AMINO-ACID SEQUENCE α-PsGal, AND METHOD FOR ITS PRODUCTION |
2012 |
- Balabanova Larisa Anatol'Evna
- Golotin Vasilij Aleksandrovich
- Bakunina Irina Jur'Evna
- Rasskazov Valerij Aleksandrovich
|
RU2504583C1 |
