FIELD: genetic engineering.
SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.
EFFECT: invention can be used for producing mature human apolipoprotein A-I.
3 cl, 1 tbl, 5 dwg, 7 ex
| Title |
Year |
Author |
Number |
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2017 |
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2011 |
|
RU2531524C2 |
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2021 |
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2022 |
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RU2798545C1 |
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2002 |
- Padkina M.V.
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|
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2012 |
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2006 |
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