METHOD FOR ACCUMULATION OF POLYHYDROXYALKANOATES IN BIOMASS WITH ON-LINE MONITORING (VERSIONS) Russian patent published in 2014 - IPC C12P7/62 C02F3/30 C02F9/00 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Disclosed are fed methods of producing high-molecular polyhydroxyalkanoates (PHA) in biomass (versions). The method comprises directing biomass into a reactor having at least one stimulating zone with a high average substrate concentration and at least one maintenance zone with a lower average substrate concentration; intermittently and repeatedly feeding an organic carbon-containing substrate into the biomass in the at least one stimulating zone; wherein substrate concentration in the stimulating zone lies between 10 and 1000 mg-COD/l; monitoring the respiration rate of the biomass and determining substrate concentration in the maintenance zone; circulating the biomass back and forth between the stimulating zone and the maintenance zone, so that the respiration rate of the biomass in the maintenance zone does not fall by more than 70% of the extant achievable maximum respiration rate of the biomass. When the biomass accumulates the required level of PHA or when PHA saturation level is achieved, the biomass is collected from the reactor. Another version of the method uses a sludge mixture containing biomass.

EFFECT: methods enable to obtain PHA with average molecular weight of at least 400000 g/mol.

18 cl, 11 dwg