FIELD: biotechnologies.
SUBSTANCE: method for obtaining growth stimulator Listeria monocytogenes provides for incubation of a bird egg with further cooling during 6-7 days at the temperature of 2-4°C, homogenisation, filtration, centrifugation, filtration, tyndalisation during 5-6 days and packing. As bird eggs, quail eggs are used, which are incubated during 8 days; before incubation, as well as on the 3rd, 5th, 7th and 8th days of incubation eggs are ozone treated during 15 minutes with 2.1 g/m3 of an ozone-oxygen mixture. After filtration is completed, the obtained mass is diluted with distilled water in the ratio of 1:3, and then, tyndalisation is performed at the temperature of 55-59°C.
EFFECT: method allows increasing the volume of bacterial mass and improving quality of a stimulator.
2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCTION OF FETAL MICROORGANISM GROWTH STIMULATOR | 2004 |
|
RU2283347C2 |
| NUTRIENT MEDIUM FOR CULTIVATION OF LISTERIOSIS CAUSATIVE AGENT | 2012 |
|
RU2525637C2 |
| METHOD FOR STIMULATING EMBRYONIC AND POSTEMBRYONIC DEVELOPMENT OF POULTRY | 2021 |
|
RU2787185C1 |
| CULTURE MEDIUM FOR GROWING LEGIONELLA | 2009 |
|
RU2412240C1 |
| SOLID NUTRIENT MEDIUM CULTIVATION OF A PLAGUE MICROBE | 2016 |
|
RU2648153C1 |
| METHOD FOR PREPARING OF COMPLEX BIOLOGICALLY ACTIVE ADDITIVE FOR ANIMALS ON THE BASIS OF EMBRYO AND PLANT SUBSTRATES | 2006 |
|
RU2320197C1 |
| METHOD FOR PREPARING A FODDER ADDITIVE BASED UPON EMBRYONIC TISSUES AND PROBIOTICS | 2005 |
|
RU2303368C1 |
| Yersinia pseudotuberculosis GROWTH STIMULATOR | 2012 |
|
RU2502796C2 |
| METHOD FOR DISINFECTION OF HATCHING EGGS | 2020 |
|
RU2736327C1 |
| METHOD OF STORAGE OF INCUBATION DUCK EGGS | 2017 |
|
RU2658845C1 |
