CYTOLOGICAL AND HISTOLOGICAL FIXING COMPOSITION AND STAINING METHOD Russian patent published in 2014 - IPC G01N1/30 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to a composition for the fixation of tissues and/or cells, and/or cell structures on the microscope slides in order to stain them and analyse under the microscope or by a system for an image analysis, to the application of the said composition and versions of the method of its obtaining, as well as to versions of the method of staining structures with its application. The composition contains at least the following compounds (percent content is given for the complete composition weight): from 40 to 60% of ethanol and/or isopropanol, dimethylsulphoxide, from 0.1 to 1% of ethyleneglycol, from 2 to 12% of water and from 0.1 to 0.5% of sodium chloride. The method of the composition obtaining includes at least mixing ethanol and/or isopropanol with ethyleneglycol to obtain a solution 1, dissolution of sodium chloride in water to obtain solution 2, addition of solution 2 into solution 1 with mixing with obtaining solution 3, after that, addition of dimethylsulphoxide and filtration. The method can also include a stage of preparing solution 4, containing at least, dimethylsulphoxide, blue dye and red dye, which is then mixed with solution 3. The method of staining cells or cell structures, in particular for blood and bone marrow, includes at least contact with a preparation to be stained with the said fixing composition, and the contact time can constitute 5-8 minutes. After that, contact of the fixed preparation with a buffer solution, pH of which constitutes from 6.5 to 7.0, is realised. It is also possible to use in a staining buffer solution with pH from 6.8 to 7.2, with the time of the fixed preparation contact with such a solution constituting 2-3 minutes. After that, contact of the preparation with a washing solution is realised, and the time of contact with the washing solution can constitute 5-20 seconds.

EFFECT: effective fixation of the cells in order to perform their staining to provide a possibility of obtaining reproducible and stable results.

13 cl, 2 ex, 16 dwg