METHOD OF SCREENING AND MONITORING CANCEROUS DISEASES AND KIT THEREFOR (VERSIONS) Russian patent published in 2014 - IPC C12Q1/68 C12P19/34 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biochemistry. Disclosed are versions of a method of screening and monitoring cancerous diseases, which includes collecting tissue samples, extracting RNA from the tissue sample, synthesis of cDNA, amplification by multiple reverse transcription polymerase chain reaction, followed by analysis of the amplified products. The polymerase chain reaction is carried out using a primer composition with sequences SEQ ID NO: 1-24 and 26-29, which are specific to mRNA of genes MAGEA1-6, GAGE1-8, NY-ESO-1, SSX1, 2, 4, XAGE1, TRAG3 and MAGEC1. Analysis of the amplified products is also carried out using probes represented by sequences SEQ ID NO:30-35 and 37. Also disclosed are versions of a kit for screening and monitoring cancerous diseases. The kit includes primers with sequences SEQ ID NO: 1-24 and 26-29, and can further include probes with sequences SEQ ID NO: 30-35 and 37.

EFFECT: invention increases accuracy of diagnosing cancerous diseases of different body parts.

7 cl, 3 dwg, 2 tbl