FIELD: biotechnology.
SUBSTANCE: invention relates to production of the recombinant human proteins and can be used for the recombinant human GAGE1 protein preparation and purification. Method of the recombinant human protein GAGE1 producing consists of creating a pGAGE1 recombinant plasmid based on pET-30a, expression of the recombinant protein and its chromatographic purification and subsequent renaturation and sterilizing filtration.
EFFECT: invention makes it possible to produce the recombinant human protein GAGE1 with the purified protein yield of 24,5 mg / L E. coli of BL21 (DE3) pLysS strain.
1 cl, 3 dwg
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