FIELD: chemistry.
SUBSTANCE: invention relates to biochemistry, in particular to molecular biology, biotechnology, genetic engineering. The invention represents optimised DNA sequences, coding a light and heavy chain of a monoclonal antibody, intended for the synthesis of the antibody in recombinant CHO-S cells, and a method of obtaining the said antibody in the said cells. The performed work resulted in the elaboration of several conditions making it possible to obtain the monoclonal antibody with high productivity.
EFFECT: invention makes it possible to maximise the cell productivity and optimise post-translational modifications of the antibody, in particular glycosylation of heavy chains of the antibody.
3 cl, 2 dwg, 6 tbl
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| HUMANISED ANTIBODY TO TNF-α, ITS ANTIGEN-BINDING FRAGMENT (Fab) AND THEIR APPLICATION | 2011 |
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RU2556815C2 |
| MONOCLONAL ANTIBODIES AND METHODS OF USING THEM | 2017 |
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RU2698048C2 |
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RU2790232C2 |
| ANTI-MIF ANTIBODIES | 2008 |
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RU2509777C2 |
