FIELD: medicine.
SUBSTANCE: invention can be used for the rapid analysis of cholesterol in immune complexes (CICs). Precipitated immune complexes containing multiply modified low-density lipoproteins of human blood serum are prepared by processing in buffer containing 10% PEG 3350, in ratio 1:3, incubating for 10 min at room temperature. The precipitated CICs are separated by centrifugation at 3100 g for 10 min at 23°C, diluted in PEG-free buffer; cholesterol is measured with the use of an enzymatic kit, and if the CICs content is more than 8.4 mg/dl, a high level is stated.
EFFECT: invention enables providing more accurate quantification of the CICs, conducting the wide pre-clinical screening examinations for atherosclerosis detection, and controlling the clinical effectiveness.
2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINATION OF ATHEROGENICITY OF IMMUNE COMPLEXES | 2013 |
|
RU2549467C1 |
| METHOD FOR RAPID DETERMINATION OF CHOLESTEROL IN IMMUNE COMPLEXES | 2012 |
|
RU2530627C2 |
| INSTANT METHOD FOR ASSESSING ATHEROGENICITY OF IMMUNE COMPLEXES OF HUMAN BLOOD SERUM | 2013 |
|
RU2549466C1 |
| METHOD FOR SELECTION AND STUDY OF ATHEROGENICITY OF IMMUNE COMPLEXES CONTAINING MULTIPLE MODIFIED LIPOPROTEINS | 2016 |
|
RU2632118C1 |
| METHOD AND TEST SYSTEM FOR DETECTION OF CIRCULATING IMMUNE COMPLEXES | 2010 |
|
RU2452962C2 |
| METHOD FOR SEPARATION AND QUANTITATIVE DETERMINATION OF MULTIPLY MODIFIED LOW-DENSITY LIPOPROTEINS | 2015 |
|
RU2592238C1 |
| METHOD TO DETERMINE ATHEROGENICITY OF HUMAN BLOOD | 2012 |
|
RU2497116C1 |
| MEDIUM AND METHOD FOR DETECTION OF MULTIPLY MODIFIED BLOOD SERUM LIPOPROTEINS | 2010 |
|
RU2444014C1 |
| METHOD FOR DETERMINING LYSIS MULTIPLE MODIFIED LOW DENSITY LIPOPROTEIN | 2015 |
|
RU2577719C1 |
| METHOD FOR RAPID DETERMINATION OF RESISTANCE TO LDL OXIDATION OF HUMAN SERUM | 2015 |
|
RU2578027C1 |
