FIELD: medicine.
SUBSTANCE: invention can be used for the instant assessment of atherogenicity of the immune complexes (IC) of human blood serum. Precipitated ICs from human blood serum are prepared by treating with a buffer containing 10% polyethylene glycol of molecular weight 3,350 (PEG-3350) in ratio 1:3.5, incubating for 10 min at room temperature. Aggregated ICs are deposited by centrifuging and dissolved in PEG-3350 free buffer; the immune complex cholesterol (ICC) is measured, and a guinea pig's complement fixation (CF) by the precipitated immune complexes is determined. The IC atherogenicity is calculated as CF/ICC relation, and if the derived value is less than 4 units, the high blood atherogenicity is stated.
EFFECT: higher assessment accuracy.
1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINATION OF ATHEROGENICITY OF IMMUNE COMPLEXES | 2013 |
|
RU2549467C1 |
| METHOD FOR RAPID ANALYSIS OF CHOLESTEROL IN IMMUNE COMPLEXES | 2013 |
|
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| METHOD FOR RAPID DETERMINATION OF CHOLESTEROL IN IMMUNE COMPLEXES | 2012 |
|
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| METHOD AND TEST SYSTEM FOR DETECTION OF CIRCULATING IMMUNE COMPLEXES | 2010 |
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| MEDIUM AND METHOD FOR DETECTION OF MULTIPLY MODIFIED BLOOD SERUM LIPOPROTEINS | 2010 |
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| METHOD FOR INSTANT DETERMINATION OF BLOOD ATHEROGENICITY (VERSIONS) | 2010 |
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| METHOD OF DETERMINATION OF CIRCULATING IMMUNE COMPLEXES | 2005 |
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| METHOD FOR ASSESSING CHARACTER OF AUTOIMMUNE REACTION OF HUMAN BODY TO MULTIPLE MODIFIED LOW-DENSITY LIPOPROTEINS IN LYTIC TEST | 2017 |
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RU2680848C1 |
