FIELD: chemistry.
SUBSTANCE: invention relates to field of biochemistry, in particular to polypeptide, possessing amylase activity, derived from parent polypeptide, which represents non-maltogenic exoamylase of wild type, which has at least 90% identity SEQ ID NO: 13. Said polypeptide contains amino acid substitution in position 307 for lysine (K) or arginine (R), in accordance with numeration of positions of Pseudomonas saccharophilia exoamylase sequence, represented in SEQ ID NO: 1. Polypeptide is applied as food or forage additive, in method of starch processing to obtain starch-containing food or forage product, in particular bakery product.
EFFECT: invention makes it possible to increase thermal stability and exospecificity of non-maltogenic exoamylase in comparison with wild type polypeptide.
62 cl, 14 dwg, 25 tbl, 37 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYPEPTIDE | 2005 |
|
RU2415939C2 |
| VERSIONS OF GLUCOAMYLASE | 2006 |
|
RU2439153C2 |
| VARIANT OF MALTOGENIC α -AMYLASE | 1999 |
|
RU2258739C2 |
| METHOD FOR STARCH TREATMENT | 2003 |
|
RU2315811C2 |
| CLEANING COMPOSITIONS CONTAINING VERSIONS OF AMYLASE IN ACCORDANCE WITH LIST OF SEQUENCES | 2012 |
|
RU2617954C2 |
| LIPOLYTIC ENZYME FOR USE IN BAKING | 2018 |
|
RU2763469C2 |
| VERSIONS OF FAMILY CONSISTING OF 44 XYLOGLUCANASES | 2009 |
|
RU2545721C2 |
| VERSIONS OF ALPHA-AMYLASE WITH CHANGED PROPERTIES | 2008 |
|
RU2499044C2 |
| POLYPEPTIDES EXHIBITING ANTIMICROBIAL ACTIVITY AND CODING POLYNUCLEOTIDES | 2006 |
|
RU2415150C2 |
| POLYPEPTIDES HAVING ANTIMICROBIAL ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME | 2006 |
|
RU2512525C2 |
