METHOD OF PRODUCTION OF PROPIONYLCHOLINESTERASE Russian patent published in 2015 - IPC C12N9/14 B01D15/38 

FIELD: biotechnology.

SUBSTANCE: ganglia of cephalopods are homogenised by double sonication. The obtained homogenate is diluted with distilled water, the mixture is filtered, centrifuged and the supernatant is passed through a chromatography column, where the sorbent is used as concanavalin A-sepharose (Con-A-sepharose), and dried, and prior to drying the propionylcholinesterase the solution of polyethylene glycol is added to the enzyme solution. The enzyme solution is sublimated at a temperature of final drying not exceeding 30°C.

EFFECT: obtaining propionylcholinesterase with optimal specific activity on industrial scale.

2 cl, 1 ex