METHOD FOR ASSESSING TOXICITY HAZARD OF ANTICHOLINESTERASE COMPOUNDS Russian patent published in 2014 - IPC G01N33/48 G01N33/52 

FIELD: medicine.

SUBSTANCE: summary of declared method consists in determining the anticholinesterase effect of solutions of the examined anticholinesterase compounds (AnCEC) of a preparation of cholinesterase - the enzyme propionyl cholinesterase of calamari brain tissue (PCE), namely in estimating a percentage PCE activity inhibition in solutions of low (0.04 U/ml) and high (4 U/ml) concentrations. A toxicity hazard of the examined AnCEC solutions is assessed by a difference of their effect on PCE activity in the solutions of low and high concentrations. If a percentage of PCE activity inhibition in the solution of high concentration goes to zero or does not exceed 5-10%, the examined solution is stated to contain high-toxicity AnCEC of the 1^st hazard class. If the inhibitory effect size remains constant or falls by no more than 10%, the presence of low-toxicity AnCEC is stated.

EFFECT: using the declared method enables assessing the toxicity hazard of the anticholinesterase compounds for 30-60 minutes in various situations, where their composition is unknown or they present in the form of solutions of an unknown concentration.

1 tbl, 2 ex