CHROMATOGRAPHIC LIGAND Russian patent published in 2015 - IPC C07C211/27 C07C211/08 C07C215/28 C07C215/90 B01D15/38 C07K16/00 C07K1/22 C07K1/18 C07K17/06 

FIELD: chemistry.

SUBSTANCE: matrix can be used in purification of proteins, where protein represents antibody, fragment of antibody or antibody-containing fused protein. Ligand corresponds to the following formula (I): R₁ -R ₂-N(R₃)-R₄ -R ₅ , where R₁ represents non-substituted phenyl group; R₂ represents hydrocarbon chain, containing 0-4 carbon atoms, preferably 1-4 carbon atoms; R₃ represents hydrocarbon chain, containing 1-3 carbon atoms; R₄ represents hydrocarbon chain, containing 1-5 carbon atoms; and R₅ represents OH or H. As base matrix contains particles, in fact representing spherical particles, or has membranous or porous structure. Method of obtaining separation matrix includes immobilisation of said ligand on base mainly through amine group. Obtained matrix is placed into chromatographic column and after that sterilised if necessary. In order to separate one or more antibodies from one or more other compounds in liquid sample mobile phase, containing said antibodies and compound(s), are brought into contact with separation matrix. Liquid sample can contain supernatant, obtained in cell fermentation or unprocessed nutritional substance. In the process of application of chromatographic column mobile phase passes through column under impact of gravity and/or rocking, and antibodies are obtained in flow liquid of column. Invention also described set for purification of antibodies from one or more other components in liquid, containing in separate compartments chromatographic column, filled with separation matrix, one or more than one buffer and written instructions.

EFFECT: claimed invention relates to novel separation matrix, containing ligand, bound to base.

20 cl, 6 dwg, 4 tbl, 4 ex