FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and particularly to a method of purifying G-CSF (granulocyte-colony stimulating factor) in a purification sequence using chromatography. The method includes performing chromatography at least once using a multimodal resin with a negatively charged ligand in the form of 2-(benzoylamino)butanoic acid. G-CSF is bound with the multimodal resin at pH in the range of 4-6.2. The G-CSF is eluted at pH higher than 6.3 or with an elution agent in an elution buffer, where said elution agent includes an amino acid having a backbone chain and/or high ionic force in the elution buffer selected from a group consisting of arginine, lysine and/or histidine. Concentration of the elution agent is in the range of about 0.1 M to about 2.0 M.
EFFECT: present invention enables to obtain purified G-CSF with high output.
14 cl, 14 dwg, 10 tbl, 11 ex
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