METHOD OF OBTAINING TEST-SYSTEM FOR DIAGNOSTICS OF CYTOMEGALOVIRUS INFECTION BY METHOD OF IMMUNOBLOTTING Russian patent published in 2015 - IPC G01N33/531 G01N33/569 

FIELD: medicine.

SUBSTANCE: group of inventions represents a method of obtaining a test-system for the diagnostics of the cytomegalovirus infection, including obtaining a purified viral lysate, preparation of an immunosorbent, containing specific CMV proteins pp 150, pp 130, gp 75, pp 65, gp 55, pp52, p 38, pp 28, preparation of solutions for electrophoresis, preparation of a solution for dilution of serums, preparation of a 5-fold concentrate of a washing solution, preparation of a conjugate, packing of the test-system, as well as the test-system, obtained by the said method. Application of a natural lysate highly-purified antigen of the cytomegalovirus strain "CMV AD 169" in obtaining the immunosorbent and the presence in it of a complete spectrum of the CMV specific proteins (in total 8 proteins), has the following advantages: the analysis sensitivity increases considerably, the setting time decreases, the necessity of preliminary dilution of the samples is absent, an initial stage of immunosorbent blocking is absent, more convenient packing considerably reduces the analysis time.

EFFECT: method is referent, maximum highly sensitive, highly specific, confirming the diagnosis in case of suspicion of the infection if positive or doubtful results are obtained.

4 cl, 6 tbl