FIELD: medicine.
SUBSTANCE: invention represents a method for preparing an immunosorbent for diagnosing herpes simplex type I virus involving preparing a polymer solid-phase carrier adsorbing a herpes type I virus antigen, aqueous 20% polyethylene glycol (PEG-20), adsorbing on the carrier in 0.2M carbonate-bicarbonate buffer at pH 9.6 of the lysate high-purity herpes type 1 virus antigen prepared of the herpes simplex type I virus strain - ATCC VR-539, washing, lyophilisation, incubation of the sorbed antigens with a blocking solution. Using the natural lysate high-purity herpes type I virus antigen ATCC-VR-539 enables providing considerably higher analysis sensitivity, tris-based blocking system ensures neutralising the undesired non-specified reactions. Besides, using the invention leads to better conditions of the agent activity on the surface of the solid-phase carrier, reducing the agent consumption.
EFFECT: using polyethylene glycol in plate processing enables higher specificity and sensitivity of the kit.
1 tbl, 1 ex
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