METHOD FOR OBTAINING CALLUS CULTURE HEDYSARUM ALPINUM L. Russian patent published in 2023 - IPC C12N5/04 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular to the production and cultivation of cell cultures of medicinal plants, and can be used in the pharmaceutical industry to obtain raw materials representing the callus culture of Hedysarum alpinum L., in order to isolate a complex of biologically active substances. The objective of the present invention is to develop a method for obtaining a callus culture of Hedysarum alpinum L., promising as a raw material for the isolation of biologically active substances and the creation of medicines based on them. The task is solved by obtaining a cell culture from 3-week-old sterile seedlings dissected into explants and placed on an agarized MS nutrient medium with the addition of 1 mg/l 2-4 D and 0.1 mg/l 6-BAP for callus formation and further cultivation of the resulting callus on an agarized MS nutrient medium with the addition of 2 mg/l α-NAC and 0.5 mg / l 6-BAP in the absence of light, temperature 26 ±1 °C and 70% humidity.

EFFECT: present invention enables to produce a callus culture of Hedysarum alpinum L., characterized by a significant increase in biomass and a high specific growth rate.

1 cl, 1 dwg, 2 tbl, 1 ex