FIELD: biotechnologies.
SUBSTANCE: invention refers to a method of simultaneous determination of zinc cation concentration and zinc and copper cation ratio in blood serum, involving use of dithizone solution in carbon tetrachloride as the main reactant, incubation at room temperature in alkali medium, organic phase removal and colorimetry of colloid solutions of blood serum sample and standard solution at 535 nm, zinc cation concentration calculation, colorimetry of colloid solutions of blood serum sample and standard solution at 435 nm and molar ration calculation for zinc and copper cations.
EFFECT: determination of zinc cation concentration in blood serum together with zinc and copper cation ratio in the same sample without additional reactants.
4 ex
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Authors
Dates
2015-04-20—Published
2013-06-25—Filed