FIELD: medicine.
SUBSTANCE: invention refers to biotechnology and immunology. What is described is a method for producing a preparation of an antibody or its antigen-binding portion with a lower content of host cell protein (HCP). The method provides reducing a pH value to approximately 3.8 in the sample by adding citric or phosphoric acid, reducing the pH value to 5.0, applying the sample onto ion-exchange resin, collecting the sample after the ion exchange, applying the sample onto resin for performing a hydrophobic-interaction chromatography and collecting a post-chromatography antibody preparation. The invention can be used in medicine.
EFFECT: method enables producing the antibody preparation, which contains the reduced viral particle count and possesses the reduced viral activity.
22 cl, 5 dwg, 11 tbl
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