SEPARATION AND PURIFICATION OF ANTIBODIES WITH APPLICATION OF PROTEIN A-BASED AFFINITY CHROMATOGRAPHY Russian patent published in 2014 - IPC C07K1/16 C07K16/00 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and represents a method of obtaining a preparation of an antibody or its antigen-binding site with reduced content of host cell proteins (HCP) from a sample mixture. The claimed invention can be used to obtain the antibody preparation. The method includes bringing pH to values 3-4 to form an initial separated sample. After that, pH of the initial separated sample is brought to values 6-8. The initial separated sample is brought in contact with resin for protein A-based affinity chromatography. The resin for affinity chromatography is washed out with a buffer solution. Sampling is carried out after affinity chromatography. The sample after affinity chromatography is brought in contact with an ion-exchange resin, after ion-exchange sampling is carried out. After the ion exchange sample is brought in contact with the resin for hydrophobic interaction chromatography (HIC) with sampling after HIC.

EFFECT: invention makes it possible to obtain the preparation of the antibody or its antigen-binding site with reduced HCP content.

25 cl, 12 dwg, 8 tbl, 4 ex