FIELD: biotechnology.
SUBSTANCE: method comprises immunoaffinity chromatography using mini-antibodies a-hLF-1 and a-hLF-4, which amino acid sequences are presented as SEQ ID NO:1 and SEQ ID NO:2. The invention may be used to obtain highly purified fraction of human lactoferrin.
EFFECT: invention enables to carry out with high efficiency the separation of proteins of lactoferrin of human and goat consisting in milk, using the single-domain mini-antibodies.
6 dwg, 2 ex
Title | Year | Author | Number |
---|---|---|---|
GENETIC CONSTRUCTS LTF3, LTF5, LTF7, LTF10, LTF11 FOR PRODUCING RECOMBINANT HUMAN LACTOFERRIN (VERSIONS) | 2011 |
|
RU2491343C1 |
GENETIC DESIGN FOR EXPRESSION OF THE FUNCTIONALLY-ACTIVE HUMAN STRESS-PROTEIN (HPS70) WITH MUTATED GLYCOSILATION SITES FOR WORKING IN EUCARIOTIC EXPRESSION SYSTEMS | 2016 |
|
RU2647570C1 |
RECOMBINANT SINGLE-DOMAIN ANTIBODIES SPECIFICALLY BINDING HUMAN INTERLEUKIN-6, METHOD FOR THEIR PREPARING AND USING FOR DETECTION OF THIS PROTEIN | 2015 |
|
RU2603269C1 |
POLYPEPTIDE MODULE FOR BINDING CONSERVATIVE EPITOPE OF RECEPTOR-BINDING DOMAIN OF SPIKE PROTEIN OF SARS-CoV-2 CORONAVIRUS | 2022 |
|
RU2809183C1 |
RECOMBINANT SINGLE-DOMAIN ANTIBODIES SPECIFICALLY BINDING F4/80 PROTEIN, METHOD FOR PRODUCTION THEREOF AND USE FOR DETECTION OF SAID PROTEIN | 2015 |
|
RU2599423C1 |
SINGLE-DOMAIN ANTIBODY SPECIFICALLY BINDING HUMAN ERBB3 RECEPTOR, WITHOUT CANONICAL DISULPHIDE BOND | 2018 |
|
RU2721854C1 |
EXPRESSION VECTOR BASED ON ADENO-ASSOCIATED VIRUS CARRYING RECOMBINANT ANTIBODY GENES AND ITS USE FOR PREVENTION OF DISEASES CAUSED BY INFLUENZA A VIRUS AND SARS-CoV-2 VIRUS | 2023 |
|
RU2817792C1 |
ANTIBODIES FOR BINDING OF IMIGLUCERASE AND USE THEREOF IN AFFINITY CHROMATOGRAPHY | 2017 |
|
RU2676956C1 |
SINGLE-DOMAIN ANTIBODY FOR NEUTRALIZING VIRUSES AND ITS MODIFICATION, AND A METHOD FOR THEIR USE FOR EMERGENCY PREVENTION OF DISEASES CAUSED BY INFLUENZA A VIRUS | 2021 |
|
RU2777073C1 |
GENETIC STRUCTURE RBCT70 FOR EXPRESSION OF THE MAIN HUMAN STRESS PROTEIN IN MILK OF TRANSGENIC ANIMALS | 2015 |
|
RU2644663C2 |
Authors
Dates
2015-06-20—Published
2014-02-03—Filed