METHOD OF SEPARATING LACTOFERRIN OF HUMAN AND GOAT BY MEANS OF DIFFERENTIAL IMMUNOAFFINITY CHROMATOGRAPHY USING SINGLE-DOMAIN MINI-ANTIBODIES Russian patent published in 2015 - IPC C12P21/02 G01N33/563 C07K16/18 C12N15/12 

Abstract RU 2553515 C1

FIELD: biotechnology.

SUBSTANCE: method comprises immunoaffinity chromatography using mini-antibodies a-hLF-1 and a-hLF-4, which amino acid sequences are presented as SEQ ID NO:1 and SEQ ID NO:2. The invention may be used to obtain highly purified fraction of human lactoferrin.

EFFECT: invention enables to carry out with high efficiency the separation of proteins of lactoferrin of human and goat consisting in milk, using the single-domain mini-antibodies.

6 dwg, 2 ex

Similar patents RU2553515C1

Title Year Author Number
GENETIC CONSTRUCTS LTF3, LTF5, LTF7, LTF10, LTF11 FOR PRODUCING RECOMBINANT HUMAN LACTOFERRIN (VERSIONS) 2011
  • Georgieva Sofija Georgievna
  • Georgiev Georgij Pavlovich
  • Gol'Dman Igor' L'Vovich
  • Krasnov Aleksej Nikolaevich
  • Sadchikova Elena Rubenovna
RU2491343C1
GENETIC DESIGN FOR EXPRESSION OF THE FUNCTIONALLY-ACTIVE HUMAN STRESS-PROTEIN (HPS70) WITH MUTATED GLYCOSILATION SITES FOR WORKING IN EUCARIOTIC EXPRESSION SYSTEMS 2016
  • Evgenev Mikhail Borisovich
  • Garbuz David Grigorevich
  • Georgieva Sofiya Georgievna
  • Karpov Vadim Lvovich
  • Krasnov Aleksej Nikolaevich
  • Gurskij Yaroslav Georgievich
RU2647570C1
RECOMBINANT SINGLE-DOMAIN ANTIBODIES SPECIFICALLY BINDING HUMAN INTERLEUKIN-6, METHOD FOR THEIR PREPARING AND USING FOR DETECTION OF THIS PROTEIN 2015
  • Tillib Sergej Vladimirovich
  • Nedospasov Sergej Arturovich
  • Efimov Grigorij Aleksandrovich
  • Drutskaja Marina Sergeevna
  • Kruglov Andrej Alekseevich
  • Gorjajnova Oksana Sergeevna
  • Ivanova Tatjana Ilinichna
RU2603269C1
POLYPEPTIDE MODULE FOR BINDING CONSERVATIVE EPITOPE OF RECEPTOR-BINDING DOMAIN OF SPIKE PROTEIN OF SARS-CoV-2 CORONAVIRUS 2022
  • Tillib Sergej Vladimirovich
  • Vorobev Pavel Olegovich
RU2809183C1
RECOMBINANT SINGLE-DOMAIN ANTIBODIES SPECIFICALLY BINDING F4/80 PROTEIN, METHOD FOR PRODUCTION THEREOF AND USE FOR DETECTION OF SAID PROTEIN 2015
  • Tillib Sergej Vladimirovich
  • Nedospasov Sergej Arturovich
  • Efimov Grigorij Aleksandrovich
  • Rozov Fedor Nikolaevich
  • Goryajnova Oksana Sergeevna
  • Ivanova Tatyana Ilinichna
  • Rutovskaya Marina Vladimirovna
RU2599423C1
SINGLE-DOMAIN ANTIBODY SPECIFICALLY BINDING HUMAN ERBB3 RECEPTOR, WITHOUT CANONICAL DISULPHIDE BOND 2018
  • Yudenko Anna Nikolaevna
  • Eliseev Igor Evgenevich
  • Ukrainskaya Valeriya Mikhajlovna
  • Chakchir Oleg Borisovich
RU2721854C1
EXPRESSION VECTOR BASED ON ADENO-ASSOCIATED VIRUS CARRYING RECOMBINANT ANTIBODY GENES AND ITS USE FOR PREVENTION OF DISEASES CAUSED BY INFLUENZA A VIRUS AND SARS-CoV-2 VIRUS 2023
  • Riabova Ekaterina Igorevna
  • Derkaev Artem Alekseevich
  • Dovgii Mikhail Andreevich
  • Esmagambetov Ilias Bulatovich
  • Shchebliakov Dmitrii Viktorovich
  • Smirnova Ekaterina Alekseevna
  • Khossain Roza Makhbubovna
  • Prokofev Vladimir Vladimirovich
  • Favorskaia Irina Alekseevna
  • Grafova Anastasiia Sergeevna
  • Efimova Viktoriia Viacheslavovna
  • Byrikhina Daria Valerevna
  • Gosudarev Andrei Igorevich
  • Evgrafova Elina Alekseevna
  • Shmarov Maksim Mikhajlovich
  • Dolzhikova Inna Vadimovna
  • Shevlyagina Natalya Vladimirovna
  • Andreevskaya Svetlana Georgievna
  • Zhukhovitskij Vladimir Grigorevich
  • Naroditskij Boris Savelevich
  • Logunov Denis Yurevich
  • Gintsburg Aleksandr Leonidovich
RU2817792C1
ANTIBODIES FOR BINDING OF IMIGLUCERASE AND USE THEREOF IN AFFINITY CHROMATOGRAPHY 2017
  • Anisimov Roman Lvovich
  • Simonov Vladimir Mikhajlovich
  • Katorkin Sergej Aleksandrovich
RU2676956C1
SINGLE-DOMAIN ANTIBODY FOR NEUTRALIZING VIRUSES AND ITS MODIFICATION, AND A METHOD FOR THEIR USE FOR EMERGENCY PREVENTION OF DISEASES CAUSED BY INFLUENZA A VIRUS 2021
  • Shcheblyakov Dmitrij Viktorovich
  • Voronina Darya Vladimirovna
  • Esmagambetov Ilyas Bulatovich
  • Derkaev Artem Alekseevich
  • Shcherbinin Dmitrij Nikolaevich
  • Popova Olga
  • Favorskaya Irina Alekseevna
  • Ryabova Ekaterina Igorevna
  • Zubkova Olga Vadimovna
  • Shmarov Maksim Mikhajlovich
  • Naroditskij Boris Savelevich
  • Logunov Denis Yurevich
  • Gintsburg Aleksandr Leonidovich
RU2777073C1
GENETIC STRUCTURE RBCT70 FOR EXPRESSION OF THE MAIN HUMAN STRESS PROTEIN IN MILK OF TRANSGENIC ANIMALS 2015
  • Evgenev Mikhail Borisovich
  • Garbuz David Grigorevich
  • Georgieva Sofiya Georgievna
  • Karpov Vadim Lvovich
  • Krasnov Aleksej Nikolaevich
  • Soshnikova Nataliya Valerevna
  • Dejkin Aleksej Vasilevich
  • Gurskij Yaroslav Georgievich
RU2644663C2

RU 2 553 515 C1

Authors

Tillib Sergej Vladimirovich

Privezentseva Marija Ehduardovna

Ivanova Tat'Jana Il'Inichna

Vasil'Ev Lev Anatol'Evich

Gurskij Jaroslav Georgievich

Gol'Dman Igor' L'Vovich

Sadchikova Elena Rubenovna

Georgiev Georgij Pavlovich

Dates

2015-06-20Published

2014-02-03Filed