METHOD OF PURIFICATION OF RECOMBINANT GRANULOCYTE COLONY-STIMULATING HUMAN FACTOR Russian patent published in 2012 - IPC C12P21/02

Abstract RU 2440417 C1

FIELD: medicine.

SUBSTANCE: described is method of purification of recombinant granulocyte colony-stimulating factor (filgrastim) of human. Method characteristics: at the stage of purification from coarse pollutions applied is sorbent SP-Sepharose Fast Flow, elution is carried out by gradient 0.5 M NaCl in acetate buffer pH 5-5.5, stage of fine purification is carried out by ion-changeable chromatography on sorbent YMC BioPro S30, at the stage of purified product concentration applied is sorbent CM-Sepharose Fast Flow, and elution is carried out by gradient 0.5 M NaCl in acetate buffer pH 5-5.5, additionally carried out is stage of gel-filtration by sorbent Sephadex G25, elution is carried out by 13.3 mM sodium-acetate buffer, pH 3.9.

EFFECT: invention makes it possible to considerably reduce waste of protein during purification.

2 tbl, 2 ex

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RU 2 440 417 C1

Authors

Grigor'Ev Pavel Igorevich

Selishchev Stepan Vladimirovich

Dates

2012-01-20—Published

2011-01-25—Filed