SET OF SYNTHETIC OLIGONUCLEOTIDES FOR DETECTING NUCLEOTIDE SEQUENCE OF CODING PORTION OF DES GENE AND IDENTIFYING MUTATIONS ASSOCIATED WITH DESMIN-RELATED CARDIOMYOPATHIES Russian patent published in 2015 - IPC C12N15/11 C12N15/113 C12Q1/68 

Abstract RU 2556832 C2

FIELD: medicine.

SUBSTANCE: invention refers to molecular biology and can be used in diagnosing cardiomyopathies of various origin Presented is a set of synthetic oligonucleotides for identifying mutations of a coding part of desmin (DES) gene associated with cardiomyopathies. The mutations are identified by detecting a full sequence of the coding part of DES gene. The coding part of DES gene is amplified by means of 7 pairs of synthetic oligonucleotides at the same temperature and annealing time, and the prepared amplification products are sequenced by means of one pair of universal primers.

EFFECT: presented invention enables the sensitive and specific detection of DES gene mutations, with reducing the amplification reaction time, the number of manipulations, the time of agent application for the sequencing reaction, and reducing a probability of a reaction error.

3 cl, 1 dwg

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RU 2 556 832 C2

Authors

Kostareva Anna Aleksandrovna

Smolina Natal'Ja Aleksandrovna

Malashicheva Anna Borisovna

Urusova Marija Evgen'Evna

Kiselev Artem Mikhajlovich

Dates

2015-07-20Published

2013-06-06Filed