FIELD: molecular biology.
SUBSTANCE: described is a method for genotyping a polymorphic locus rs36210422 (G>A) of a gene by allele-specific PCR followed by electrophoresis of amplification products. Identification of allelic variants of rs36210422 (G>A) gene KCNH2 is carried out using forward and reverse primers: for allele of wild type – SEQ ID NO: 1 and SEQ ID NO: 2, for mutant – SEQ ID NO: 1 and SEQ ID NO: 3 respectively.
EFFECT: creation of a new allele-specific PCR, which enables to accurately and quickly identify a mutation NM_000238_4:c_526C>T (p_Arg176Trp) in exon 4 of the KCNH2 gene.
1 cl, 1 dwg, 1 tbl, 2 ex
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