METHOD FOR PRODUCING MULTIPLEX RICKETTSIAL DIAGNOSTIC PREPARATION Russian patent published in 2015 - IPC A61K39/02 

Abstract RU 2557951 C1

FIELD: medicine.

SUBSTANCE: method for producing a multiplex Rickettsial diagnostic preparation involves labelling Rickettsia and Coxiella corpuscular antigens with semiconductor colloidal nanoparticles of different fluorescent colours. The Rickettsia prowazekii corpuscular antigens are conjugated with the nanoparticles at a wavelength of red fluorescence (610-670 nm), and the Coxiella burnetii corpuscular antigen - with the nanoparticles at a wavelength of green fluorescence (520-540 nm). The conjugation process takes one hour and is accompanied by constant stirring. The unreacted nanoparticles are removed by Sephadex G-25 gel filtration chromatography. The labelled Rickettsia and Coxiella are combined into a multiplex diagnostic preparation to be used in the immunofluorescent nanoagglutination reaction (IFNAR). The multiplex diagnostic preparation enables detecting the anti-Rickettsia prowazekii and anti-Coxiella burnetii antibodies simultaneously within one IFNAR.

EFFECT: method simplifies the procedure of detecting the specific antibodies in human blood serum, reduces the reaction time twice and provides the more specific detection of the immunofluorescent analysis.

3 tbl, 3 ex

Similar patents RU2557951C1

Title Year Author Number
METHOD FOR PRODUCING A SET OF FLUORESCENT RICKETTSIAL AND QUERY FEVER DIAGNOSTICUMS AND USE THEREOF FOR SEROLOGICAL DIAGNOSIS OF RICKETTSIOSIS AND QUERY FEVER, A SEROLOGICAL DIAGNOSTIC TECHNIQUE 2019
  • Pantyukhina Anna Nikolaevna
  • Kostarnoj Aleksej Viktorovich
  • Kondratev Aleksej Vladimirovich
  • Gancheva Petya Gancheva
  • Gintsburg Aleksandr Leonidovich
RU2728340C1
METHOD FOR PRODUCING A FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT FOR DETECTING PATHOGENS OF RICKETTSIOSES AND COXIELLOSES, FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT AND APPLICATION THEREOF 2021
  • Pantiukhina Anna Nikolaevna
  • Kostarnoi Aleksei Viktorovich
  • Kondratev Aleksei Vladimirovich
  • Gancheva Petia Gancheva
  • Smirnova Nina Sergeevna
  • Gintsburg Aleksandr Leonidovich
RU2769578C1
0
  • Barban Petr Semenovich
  • Mirskij Valerij Yakovlevich
SU553289A1
STRAIN OF COXIELLA BURNETTI, USED FOR HYPERIMMUNE SERA PREPARATION 0
  • Tokarevich Nikolaj Konstantinovich
  • Dajter Arkadij Borisovich
  • Rybakova Nina Alekseevna
SU1724683A1
DIAGNOSTIC TECHNIQUE FOR RICKETTSIAL DISEASES GROUP OF SPOTTED FEVER, IMMUNOASSAY DIAGNOSTIC TEST SYSTEM FOR IMPLEMENTATION THEREOF 2019
  • Chekanova Tatiana Aleksandrovna
  • Kostarnoi Aleksei Viktorovich
  • Kondratev Aleksei Vladimirovich
  • Gintsburg Aleksandr Leonidovich
RU2726484C1
METHOD OF PREPARING CORPUSCULAR RICKETTSIAL SIBIRICA DIAGNOSTICUM 1994
  • Pantjukhina A.N.
  • Aleksandrova L.V.
  • Petrov V.F.
  • Tarasevich I.V.
  • Jablonskaja V.A.
RU2122427C1
STRAIN OF MAMMALIAN HYBRID CELL C3/S-3E5 OF MUS MUSCULUS L. PRODUCING MONOCLONAL ANTIBODY TO BERNET'S COXIELLAS 2003
  • Ruchko S.V.
  • Churkin I.A.
  • Lebedev V.N.
  • Panygina S.A.
  • Khamitova M.F.
  • Plekhanova T.M.
  • Jarosh A.G.
  • Kharchenko V.A.
  • Ionov S.N.
  • Maksimov V.A.
RU2257414C1
METHOD FOR DIFFERENTIATION OF RICKETTSIAE OF SPECIES RIKKETSIA AKARI FROM RIKKETSIA SIBIRICA 0
  • Rudakov Nikolaj Viktorovich
  • Shpynov Stanislav Nikolaevich
SU1756358A1
METHOD OF DRY FLUORESCENT SALMONELLOSIS DIAGNOSTICUM PREPARING 2000
  • Pantjukhina A.N.
  • Krotova M.L.
RU2175558C1
METHOD FOR SETTING DIFFERENTIAL DIAGNOSIS OF YERSINIOSIS 1992
  • Jashina N.V.
  • Dulatova M.V.
  • Fish N.G.
  • Prokopov N.I.
  • Cherkasov V.R.
  • Kravtsov Eh.G.
  • Janina T.N.
  • Gritskova I.A.
RU2087913C1

RU 2 557 951 C1

Authors

Tarasevich Irina Vladimirovna

Pantjukhina Anna Nikolaevna

Vakshtejn Maksim Sergeevich

Dezhurov Sergej Valer'Evich

Dates

2015-07-27Published

2014-05-27Filed