METHOD FOR PRODUCING MULTIPLEX RICKETTSIAL DIAGNOSTIC PREPARATION Russian patent published in 2015 - IPC A61K39/02 

FIELD: medicine.

SUBSTANCE: method for producing a multiplex Rickettsial diagnostic preparation involves labelling Rickettsia and Coxiella corpuscular antigens with semiconductor colloidal nanoparticles of different fluorescent colours. The Rickettsia prowazekii corpuscular antigens are conjugated with the nanoparticles at a wavelength of red fluorescence (610-670 nm), and the Coxiella burnetii corpuscular antigen - with the nanoparticles at a wavelength of green fluorescence (520-540 nm). The conjugation process takes one hour and is accompanied by constant stirring. The unreacted nanoparticles are removed by Sephadex G-25 gel filtration chromatography. The labelled Rickettsia and Coxiella are combined into a multiplex diagnostic preparation to be used in the immunofluorescent nanoagglutination reaction (IFNAR). The multiplex diagnostic preparation enables detecting the anti-Rickettsia prowazekii and anti-Coxiella burnetii antibodies simultaneously within one IFNAR.

EFFECT: method simplifies the procedure of detecting the specific antibodies in human blood serum, reduces the reaction time twice and provides the more specific detection of the immunofluorescent analysis.

3 tbl, 3 ex