FIELD: medicine.
SUBSTANCE: method for producing a multiplex Rickettsial diagnostic preparation involves labelling Rickettsia and Coxiella corpuscular antigens with semiconductor colloidal nanoparticles of different fluorescent colours. The Rickettsia prowazekii corpuscular antigens are conjugated with the nanoparticles at a wavelength of red fluorescence (610-670 nm), and the Coxiella burnetii corpuscular antigen - with the nanoparticles at a wavelength of green fluorescence (520-540 nm). The conjugation process takes one hour and is accompanied by constant stirring. The unreacted nanoparticles are removed by Sephadex G-25 gel filtration chromatography. The labelled Rickettsia and Coxiella are combined into a multiplex diagnostic preparation to be used in the immunofluorescent nanoagglutination reaction (IFNAR). The multiplex diagnostic preparation enables detecting the anti-Rickettsia prowazekii and anti-Coxiella burnetii antibodies simultaneously within one IFNAR.
EFFECT: method simplifies the procedure of detecting the specific antibodies in human blood serum, reduces the reaction time twice and provides the more specific detection of the immunofluorescent analysis.
3 tbl, 3 ex
Title | Year | Author | Number |
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METHOD FOR PRODUCING A SET OF FLUORESCENT RICKETTSIAL AND QUERY FEVER DIAGNOSTICUMS AND USE THEREOF FOR SEROLOGICAL DIAGNOSIS OF RICKETTSIOSIS AND QUERY FEVER, A SEROLOGICAL DIAGNOSTIC TECHNIQUE | 2019 |
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RU2728340C1 |
METHOD FOR PRODUCING A FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT FOR DETECTING PATHOGENS OF RICKETTSIOSES AND COXIELLOSES, FLUORESCENT IMMUNOGLOBULIN DIAGNOSTIC AGENT AND APPLICATION THEREOF | 2021 |
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RU2769578C1 |
0 |
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STRAIN OF COXIELLA BURNETTI, USED FOR HYPERIMMUNE SERA PREPARATION | 0 |
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Authors
Dates
2015-07-27—Published
2014-05-27—Filed