FIELD: biology, hybridoma technology.
SUBSTANCE: invention represents a new strain of mammalian hybrid cells C3/S-3E5 of Mus musculus L. producing monoclonal antibodies (MCAb) to Bernet's coxiellas (strain "Grita") in cell cultures and abdominal cavity of syngenic animals. Hybridoma C3/S-3E5 producing MCAb to this pathogen is obtained by fusion of murine myeloma of strain Sp-2/0 and murine splenocytes of strain BALB/c immunized with the concentrated and purified Bernet's coxiella preparation (strain "Grita) inactivated with formalin using polyethylene glycol of molecular mass 1000 Da as a fusing agent and the following cloning by method of maximal dilutions. Specificity of prepared MCAb: absence of cross-reactions in IFA with Provacheck's rickettsia antigen and with the non-infected accumulation substrate. Using prepared MCAb it is possible to carry out specific detection of Bernet's coxiellas by method IFA (direct and indirect variants). IFA sensitivity based on these MCAb is 2.0 x 103 ID50 x cm-3 for white rats. Applying the present invention allows detecting and identifying pathogens of rickettsial etiology.
EFFECT: improved method preparing, valuable properties of strain.
3 tbl, 1 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRID CELLS R1/S-5A6 OF ANIMALS MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODY RAISED TO RICKETTSIA PROWAZEKII | 2001 |
|
RU2198922C2 |
| STRAIN OF HYBRID CELLS M/N-10G9 OF ANIMAL MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO MARBURG VIRUS | 2001 |
|
RU2186107C1 |
| STRAIN OF T2/S-6E11 HYBRID CELLS OF ANIMAL MUS MUSCULUS L. PRODUCING MONOCLONAL ANTIBODIES RAISED TO CORONAVIRUS AS PATHOGEN OF SEVERE ACUTE RESPIRATORY SYNDROME | 2005 |
|
RU2291195C1 |
| STRAIN OF HYBRID CELLS E4/N-6 G5 OF ANIMAL MUS MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES RAISED TO EBOL'S VIRUS | 2001 |
|
RU2186106C1 |
| CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS HT 3E5 - PRODUCER OF MONOCLONAL ANTIBODIES ISOTYPE G 2A TO B- SUBUNIT OF CHOLERA TOXIN | 2015 |
|
RU2590587C1 |
| ANIMAL MUS MUSCULUS HYBRID CELL G10B6 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES | 2009 |
|
RU2401303C1 |
| ANIMAL MUS MUSCULUS HYBRID CELL F3H10 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES | 2009 |
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RU2407795C1 |
| STRAINS OF HYBRID CELLS OF ANIMALS MUS_ MUSCULUS - PRODUCERS OF MONOCLONAL ANTIBODIES TO PROTEIN GP OF EBOLA VIRUS (SUBTYPE ZAIRE) AND MONOCLONAL ANTIBODIES TO PROTEIN GP OF EBOLA VIRUS (SUBTYPE ZAIRE) | 2017 |
|
RU2686630C1 |
| STRAIN OF HYBRID CULTURED MAMMALIAN CELLS MUS MUSCULUS L, PKKK(P) 679D - PRODUCER OF MONOCLONAL ANTIBODY WITH SPECIFICITY TO PROTEIN-POLYSACCHARIDE ANTIGEN FROM YERSINIA ENTEROCOLITICA O3 AND O9 SEROVARS | 2004 |
|
RU2265051C1 |
| STRAIN OF HYBRIDOUS CULTURED CELLS MUS MUSCULUS L - A PRODUCER OF MONOCLONAL ANTIBODIES TO THE SURFACE ANTIGEN OF TYPICAL GLANDERS STRAINS PATHOGEN PSEUDOMONAS MALLEI | 0 |
|
SU1791451A1 |
