METHOD OF PRODUCTION OF DOUBLE-STRANDED RIBONUCLEIC ACID (dsRNA) FROM CELLS OF YEAST Saccharomyces cerevisiae Russian patent published in 2015 - IPC C12P19/34 C12N1/16 

FIELD: biotechnology.

SUBSTANCE: method of production of double-stranded ribonucleic acid (dsRNA) from cells of strain Saccharomyces cerevisiae RNCIM Y-448 comprises breaking the yeast cells in a buffer with pH 7.4, containing 10 mM Tris, 20 mM EDTA and 0.5 M NaCl, processing with sodium dodecyl sulphate at a concentration from 0.5 to 1.0% for 20-25 minutes at 20°C and with chloroform at a concentration of up to 25% for 20-25 minutes at 20°C. The resulting mixture is centrifuged at 6000 rev/min for 20 minutes. Concentration of dsRNA is carried out in 7-8% solution of PEG 6000 for at least 5 hours at 6°C followed by centrifugation at 6000 rev/min for 20 minutes and dissolving the resulting precipitated concentrate in water. SsRNA is separated from dsRNA in the 2 mol/l solution of LiCl for 5 h at 6°C, followed by centrifugation of the mixture at 6000 rev/min for 20 minutes, and collecting the aqueous phase. DsRNA is precipitated from the aqueous phase in 3.5 mol/l solution of LiCl for 5 h at 6°C followed by centrifugation at 6000 rev/min for 20 minutes to obtain a precipitate and dissolving it in water. Purification and precipitation of dsRNA from the solution is carried out with 55% ethanol solution. Yield of dsRNA is at least 90%, and the interferon titer in blood serum using the preparation based on the solution of dsRNA is after 24 hours 1280±60 u/ml.

EFFECT: increased yield of dsRNA higher purity and interferon-inducing activity.

2 cl, 5 dwg, 3 tbl, 6 ex