KINETIC METHOD OF DETERMINING BIOMATERIAL ANTIOXIDANT ACTIVITY Russian patent published in 2015 - IPC G01N33/48 G01N7/18 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, biology, pharmacy and food technology in application to research of homolytic properties of lipids and their participation in free-radical oxidation reactions, as well as to realisation of selection of antioxidants. Method is realised by extraction from biomaterial charge of fat-soluble antioxidant in form of heptane extract and water-soluble antioxidant in form of isopropyl extract, saturation of model substrate sample of each antioxidant with oxygen with mixing at temperature 60.0±0.2°C and determination of volume of absorbed oxygen in time by volumetric method in thermostated Warburg type installation with building graph in ΔV/t coordinates, further determination of induction period value (τ_i) from kinetic curves and calculation of the total antioxidant activity of biomaterial components in composition of model substrate taking into account control samples, which do not contain heptanes and isopropyl extract, with model substrate of fat-soluble antioxidant including biomaterial, methyl or ethyl ether of higher unsaturated fatty acids, solution of azo-bis-isobutyronitrile (AIBN) in chlorobenzene in concentration in sample (2-60)×10^-3 M; obtained sample is brought to 2 ml with chlorobenzene; model substrate of water-soluble antioxidant includes biomaterial, methyl or ethyl ether of unsaturated fatty acids, water solution of copper (II) chloride in concentration in sample (1-3)×10^-3 M, water solution of cetyltrimethylammonium bromide (CTMAB) in concentration in sample (1-5)×10^-3 M; obtained sample is brought to 4 ml with water, with determination of the total antioxidant activity of fat-soluble and water-soluble components of biomaterial being realised on the basis of specified calculated dependence.

EFFECT: increased accuracy and acceleration of determination are achieved.

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