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IPC

G01N33/535(2006-01-01)

G01N33/547(2006-01-01)

A61K39/12(2006-01-01)

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Application

2013115993/10, 2013-04-10

(24)

Start date

2013-04-10

(22)

Actual filing date

2013-04-10

(45)

Published

2015-10-20

(72)

Inventor

Dzagurova Tamara KazbekovnaTkachenko Evgenij AleksandrovichKorotina Natal'Ja AleksandrovnaSveshnikov Petr GeorgievichSolopova Ol'Ga NikolaevnaVarlamov Nikolaj Evgen'EvichMalkin Gennadij AndreevichBalovneva Marija VladimirovnaSotskova Svetlana Evgen'EvnaMikhajlov Mikhail Ivanovich

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Nauchnoe Uchrezhdenie Poliomielita I Virusnykh Ehntsefalitov Im M.P. Chumakova

METHOD OF OBTAINING IMMUNOENZYMATIC TEST SYSTEM FOR DETERMINATION OF EPITOPES OF ENVELOPE PROTEIN OF PUUMALA VIRUS WITH PROTECTIVE ORIENTATION IN VACCINE PREPARATIONS AGAINST HAEMORRHAGIC FEVER WITH RENAL SYNDROME Russian patent published in 2015 - IPC G01N33/535 G01N33/547 A61K39/12

Abstract RU 2565543 C2

FIELD: medicine.

SUBSTANCE: claimed invention relates to field of biotechnology and deals with method of obtaining immunoenzymatic test-system for determination of epitopes of envelope protein of Puumala virus with protective orientation in vaccine preparations against haemorrhagic fever with renal syndrome (HFRS). Characterised method includes obtaining murine monoclonal antibodies, possessing neutralising activity with respect to Puumala virus; sorption of said antibodies on immune panel; separation of IgG from blood serum of HFRS reconvalescents with high titres of specific antibodies to Puumala virus; preparation of conjugate of specific IgG with horse radish peroxidise.

EFFECT: claimed invention makes it possible to detect envelope protein of both live and inactivated Puumala virus with similar accuracy, which serves for control of content of said virus, which is a causative agent of haemorrhagic fever with renal syndrome, in vaccine preparation.

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RU 2 565 543 C2

Authors

Dzagurova Tamara Kazbekovna

Tkachenko Evgenij Aleksandrovich

Korotina Natal'Ja Aleksandrovna

Sveshnikov Petr Georgievich

Solopova Ol'Ga Nikolaevna

Varlamov Nikolaj Evgen'Evich

Malkin Gennadij Andreevich

Balovneva Marija Vladimirovna

Sotskova Svetlana Evgen'Evna

Mikhajlov Mikhail Ivanovich

Dates

2015-10-20—Published

2013-04-10—Filed