RECOMBINANT PLASMID DNA PMBP-PUUV_N, PROVIDING SYNTHESIS AND SECRETION OF FUSION PROTEIN MBP-PUUV_N IN ESCHERICHIA COLI CELLS, STRAIN ESCHERICHIA COLI KRX/PMBP-PUUV_N, PRODUCING SAID PROTEIN, USED TO PRODUCE RECOMBINANT PROTEIN PUUV_N USED FOR DIAGNOSIS OF HEMORRHAGIC FEVER WITH RENAL SYNDROME Russian patent published in 2025 - IPC C12N15/70 C12N15/40 C07K14/08 C07K19/00 

FIELD: biotechnology.

SUBSTANCE: disclosed is recombinant plasmid DNA pMBP-PUUV_N, which provides synthesis and secretion of the fused recombinant protein MBP-PUUV_N in E. coli cells. Also disclosed is a recombinant Escherichia coli KRX/pMBP-PUUV_N strain, obtained by transforming cells of the Escherichia coli KRX strain with said plasmid DNA pMBP-PUUV_N and producing hybrid polypeptide MBP-PUUV_N, used to produce recombinant protein PUUV_N, used for diagnosis of hemorrhagic fever with renal syndrome (HFRS).

EFFECT: invention provides synthesis of recombinant protein MBP-PUUV_N, from which the recombinant protein PUUV_N is obtained with purity of the preparation of not less than 95 % for diagnosing HFRS.

2 cl, 6 dwg, 1 tbl, 6 ex