FIELD: medicine.
SUBSTANCE: invention relates to field of biotechnology, genetic engineering and virology. In addition to SIN-vector system contains besides first helper plasmid, intended for expression of viral fusion protein gag-pol, and second helper plasmid, intended for expression of envelope protein (env) of retrovirus. First and second helper plasmids, contained in packaging cell line or applied for its short-term transfection, serve for creation of non-replicating (RCR-incompetent) viral particles. Viral particles contain RNA, which contains on 3'-end SIN-LTR, claimed in invention, where RNA can have therapeutically effective segment, which, for instance, represents transgene. For this purpose extensive deletion of U3-region, which in the process of reverse transcription is copied in 5' LTR, is provided in 3'-SIN-LTR. In addition, all coding ASLV regions, as well as retroviral splicing donor sites are removed from SIN-vector. Invention can be applied in genetic therapy.
EFFECT: viral self-inactivating (SIN) vector, based on virus of sarcoma and avian leukaemia virus (ASLV), and cleaving-packaging system are described.
24 cl, 15 dwg, 2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| VIRAL VECTORS WITH CONDITION-DEPENDENT REPLICATION AND THEIR USING | 2001 |
|
RU2301260C2 |
| MICROVESICLE AND METHOD FOR ITS PREPARATION | 2014 |
|
RU2693088C2 |
| RETROVIRAL VECTOR BASED ON MURINE LEUKOSIS VIRUS (MLV) (VERSIONS) | 1999 |
|
RU2203321C2 |
| BIOPRODUCTION OF LENTIVIRAL VECTORS | 2016 |
|
RU2729385C2 |
| IMPROVING GENETIC CONSTRUCTS FOR PROVIDING HIGH EFFICACY OF ANTI-HIV THERAPY | 2013 |
|
RU2533817C1 |
| AUXILIARY PLASMID LENTIVIRAL EXPRESSION VECTOR FOR OBTAINING HIGH TITERS OF Vpx-CONTAINING LENTIVIRAL PARTICLES, WHICH PROVIDES EFFECTIVE INFECTION OF HUMAN MONOCYTES AND DENDRITIC CELLS | 2017 |
|
RU2697781C2 |
| RECOMBINANT LENTIVIRAL VECTOR, HOST CELL TRANSDUCED WITH LENTIVIRAL VECTOR, METHODS FOR TRANSDUCTION AND USES THEREOF | 2002 |
|
RU2305708C2 |
| GENETIC MAKE-UPS FOR ANTI-HIV THERAPY | 2010 |
|
RU2426788C1 |
| RECESSIVE DIVIDING RETROVIRAL VECTOR (VARIANTS), METHOD FOR PRODUCTION THEREOF (VARIANTS), ISOLATED AND PURIFIED NUCLEIC ACID MOLECULE | 1996 |
|
RU2270250C2 |
| METHOD FOR TEMPORARY TRANSFECTION FOR PRODUCING RETROVIRUS | 2016 |
|
RU2749717C2 |
