METHOD OF DETERMINATION OF ANTILYSOZYMIC ACTIVITY OF STAPHYLOCOCCUS Russian patent published in 2015 - IPC C12Q1/02 C12R1/44 C12Q1/14 

FIELD: bioengineering.

SUBSTANCE: studied culture of staphylococcus is grown under continuous jogging, for check mixture of broth and culture without lysozyme is used, double measurement of the optical density of test and check cavities is performed after 2 and 4 hours of incubation, and level of antilysozymic activity is estimated as per degree of sign evidence at definite stock of staphylococcus calculated as per equation: , where K is coefficient of antilysozymic activity of staphylococcus; V_C is volume of broth culture of studied stock; V_L is volume of lysozyme solution of initial concentration; C_L is lysozyme concentration; M_O4 is average value of test cavities with the broth culture of the studied stock with lysozyme after 4 hours of incubation, units of optical density; M_O2 is average optical density of test cavities with the broth culture of the studied stock with lysozyme after 2 hours of incubation, units of optical density; M_C4 is average optical density of check cavities with the broth culture of the studied stock without lysozyme after 4 hours of incubation, units of optical density; M_C2 is average optical density of check cavities with the broth culture of the studied stock without lysozyme after 2 hours of incubation, units of optical density. At K<0.49 low degree of evidence of antilysozymic activity is identified, at K in range 0.5-2.49 - medium degree of evidence of antilysozymic activity, K>2.5 - corresponds to high degree of evidence of antilysozymic activity of staphylococcus.

EFFECT: this method use ensures higher accuracy of antilysozymic activity determination.

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