GLUCOOLIGASACCHARIDES, CONTAINING (ALPHA 1-4) AND (ALPHA-1-6) GLYCOSIDIC LINKAGES, THEIR APPLICATION AND METHODS OF THEIR OBTAINING Russian patent published in 2015 - IPC C12P19/04 C12P19/18 C07H3/06 C12N9/10 A23L1/522 C08B31/00 

FIELD: biotechnologies.

SUBSTANCE: method of obtaining of the mix of glucooligasaccharides containing two or more series (α1→6) glycosidic linkages and two or more series (α1→4) glycosidic linkages is offered. Poly-and/or oligosaccharide comprising on non-restorable end at least two (α-1→4)-linked units of D-glucose, are forced to contact with the enzyme α-glucanotransferase enzyme. The named enzyme is GTFB type of enzyme and splits (α1→4) and creates new (α1→4) and (α1→6) glycoside links. The released linear glucooligasaccharides and their mixes obtained by the named method, their use as fibre-prebiotic in food composition are offered. Also the genetically modified GTFA-type glucan sucrase enzyme including at least two mutations indicated in the invention formula and with GTFB-type activity is also offered. The named modified enzyme splits (α1→4) and creates new (α1→4) and (α1→6) glycosidic linkages, using as a substratum the poly-and/or the oligosaccharides containing (α1→4)-linked units of D-glucose on its non-restoring end.

EFFECT: inventions allow to obtain partially indigestible derivatives of starch.

19 cl, 2 tbl, 8 dwg