METHOD FOR OBTAINING BACTERIOPHAGE STRAIN, SPECIFIC STRAINS OF BACTERIOPHAGES AND THEIR APPLICATION Russian patent published in 2016 - IPC C12N7/00 A61K35/76 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to method for identification of bacteriophage strain, versions of bacteriophage strain and their application. Method includes obtaining collection of strains of bacteriophages, cultivation of Salmonella spp strain on sterile culture medium, application of culture samples on plate, addition of suspension of tested bacteriophage strain in concentration 6.5 x 10⁹ pfu, with ratio of bacterial suspension to suspension with phage 1:1, 1:1.5 or 1:3 and incubation at 37°C for 4 h. Resazurin is added to culture samples with further incubation in darkness at 37°C for 3 h. Colour and fluorescence of culture are controlled. Bacteriophage strain, contained in culture, preserving blue colour or not demonstrating significant increase of fluorescence in comparison with control, is identified as bacteriophage strain, specific to selected pathogen. Also claimed are bacteriophage strain PCM F/00069, bacteriophage strain PCM F/00070 and bacteriophage strain PCM F/00071 and their application for preparation obtaining.

EFFECT: group of inventions provides effective screening of bacteriophages, as well as 200 fold reduction of infection a week after termination of treatment with bacteriophage-based preparation.

8 cl, 7 dwg, 11 tbl, 3 ex