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2 822 037

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IPC

C12N7/00(2006-01-01)

C12Q1/68(2006-01-01)

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Application

2023122384, 2023-08-28

(24)

Start date

2023-08-28

(22)

Actual filing date

2023-08-28

(45)

Published

2024-06-28

(72)

Inventor

Doronin Maksim IgorevichMalygin Maksim PavlovichMikhalishin Dmitrij ValerevichChvala Ilya AleksandrovichBorisov Aleksej ValerevichProkhvatilova Larisa BorisovnaRuchnova Olga Ivanovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

METHOD FOR DIFFERENTIATION OF GENOME OF ARRIAH VACCINE STRAIN FROM FIELD ISOLATES OF RABIES VIRUS BY POLYMERASE CHAIN REACTION IN REAL TIME WITH ANALYSIS OF PEAKS OF MELTING TEMPERATURES OF AMPLICONS AND USE OF ASYMMETRIC DYE SYBR GREEN Russian patent published in 2024 - IPC C12N7/00 C12Q1/68

Abstract RU 2822037 C1

FIELD: veterinary science; virology.

SUBSTANCE: described is a method for molecular diagnostics, namely for differentiation of genome of ARRIAH vaccine strain from field isolates of rabies virus by real-time polymerase chain reaction with analysis of peaks of melting temperatures of amplicons and using asymmetric dye SYBR Green. Main advantages of the present invention are the possibility of differentiating the genome of the ARRIAH vaccine strain from field isolates of the rabies virus in a short period of time – 2.5 hours using the developed method.

EFFECT: invention is characterized by analytical specificity equal to 100%, in 95% confidence interval diagnostic sensitivity for this method is 98.74–100%, diagnostic specificity – 98.84–100%.

4 cl, 3 dwg, 5 tbl, 3 ex

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RU 2 822 037 C1

Authors

Doronin Maksim Igorevich

Malygin Maksim Pavlovich

Mikhalishin Dmitrij Valerevich

Chvala Ilya Aleksandrovich

Borisov Aleksej Valerevich

Prokhvatilova Larisa Borisovna

Ruchnova Olga Ivanovna

Dates

2024-06-28—Published

2023-08-28—Filed