FIELD: virology.
SUBSTANCE: following is described: a kit for RT-PCR to detect the genome of the rabies virus including synthetic oligonucleotide primers, complementary regions of the NP gene encoding the nucleoprotein protein of the Newcastle disease virus, “La Sota” strain, as well as an internal control sample — vaccine against Newcastle disease from “La Sota” strain. The use of this vaccine as an internal control sample in the kit is also described.
EFFECT: reduction of the risk of obtaining false negative results, the ability to use a wide range of amplifiers that meet the minimum requirements for PCR, and reduction of the time for producing an internal control sample.
2 cl, 1 tbl, 2 ex
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RU2714045C1 |
