FIELD: veterinary science; virology; molecular biology.
SUBSTANCE: described is a set of synthetic oligonucleotide primers for detecting porcine reproductive and respiratory syndrome virus of the first type in polymerase chain reaction and subsequent genotyping by genes ORF5 and ORF7.
EFFECT: creation of synthetic oligonucleotide primers complementary to the PRRS-1 genome regions, flanking the ORF7 and ORF5 genes, which enables to produce genome fragments containing the full sequences of the ORF5 and ORF7 genes of all existing genotypes in the PRRS-1, and genotyping circulating isolates by phylogenetic analysis.
1 cl, 1 dwg, 2 tbl, 5 ex
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