FIELD: medicine.
SUBSTANCE: bovine respiratory syncytial infection RNA virus is detected with the use of synthetic oligonucleotide primers complementary to a high-conserved genome region of bovine respiratory syncytial infection virus of glycoprotein F gene in polymerase chain reaction. The offered method involves DNA recovery, reverse transcription, amplification of complementary virus DNA (cDNA) on synthetic oligonucleotide primers, transport of an amplification product on gel and reaction assay. PCR is one-rounded. In positive reaction, a fragment corresponding to 381 n.s. is synthesised.
EFFECT: invention allows detecting strain and isolate DNA of bovine respiratory syncytial infection virus in sampled biomaterial of various origin at early stages of virus cultivation, and reducing cost of diagnostic procedure.
2 cl, 3 tbl, 6 ex
