METHOD FOR LIFE-TIME DETECTION OF MORPHOFUNCTIONAL CHARACTERISTICS OF CONNECTIVE TISSUE MEMBRANES OF EYEBALL IN STABLE MYOPIA ALTA Russian patent published in 2016 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: fragment of Tenon's membrane is surgically separated, ground and frozen in liquid nitrogen vapour up to -180°C. To assess CD marker distribution on the surface of Tenon's membrane cells in a flow cytofluorometer, the frozen material is unfrozen in nitrogen vapour, transferred into a centrifuge test tube, washed in normal saline and centrifuged. That is followed removing supernatant and washing once again in normal saline; the precipitate is added with normal saline; the produced tissue suspension is ground with recovering Tenon's membrane cell elements. CD markers are detected in the produced suspension, if observing an increase in CD49f+, CD49f+HLADR+, CD146+CD54-HLADR+, CD249+, CD45+CD14+HLADR+ cells and a decrease in CD45-CD14-CD44+, CD45-CD14-CD44+CD80+, CD207+, CD146+, CD146+CD54+HLADR-, CD146+CD34+ cells, stable myopia alta is diagnosed.

EFFECT: by detecting the Tenon's membrane cell subpopulations, the method makes it possible to diagnose stable myopia alta with the life-time detection of the morphofunctional characteristics of connective tissue membranes of an eyeball.

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