METHOD FOR SEARCHING TARGET PROTEINS TRIGGERING PROCESS OF CARCINOGENESIS, IN INDIVIDUAL PATIENT'S TISSUE SAMPLES FOR PURPOSES OF SUBSEQUENT ANTITUMOUR MEDICINAL TREATMENT Russian patent published in 2016 - IPC C12Q1/68 

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology, namely to a method for searching target proteins triggering the process of cancerogenesis, in individual patient's tissue samples for purposes of the subsequent antitumour therapy. A sequencing method is used to obtain transcriptomic data on gene expression levels in the samples. A kit of activated gene-control networks consisting of a central control gene and controlled genes, is produced. Differential expressed genes are derived from the transcriptomic data. The sequencing methods are used to trace variations in DNA sequences presented in the form of a set of single-nucleotide polymorphisms in the samples. Driver genes are specified among the detected genetic variations. Canonical signal pathways considerably enriched with the pre-detected central expression-control genes, differentially expressed genes, genes with identified single-nucleotide polymorphisms, and indel driver genes are searched. By means of a direct search method, the identified signal pathways are analysed to detect the components - existing proteins targeted by medicinal products for the antitumour therapy.

EFFECT: presented invention enables the high-efficacy detection of the target proteins triggering the process of cancerogenesis, in the individual patient's tissue samples.

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