FIELD: chemistry; medicine.
SUBSTANCE: invention relates to a method for preparing a composition based on modified sodium hyaluronate and use thereof in various fields of medicine, veterinary medicine and cosmetology. Method of preparing a composition based on modified copolymer of sodium hyaluronate and heparin comprises forming transverse covalent bonds between hydroxyl groups residues of D-glucuronic acid and D-N-acetylglucosamine included into composition of sodium hyaluronate, and residues of α-D-glucosamine and uronic acid related to heparin, by introducing active cross-linking bifunctional agent - (polyethylene glycol) diglycidyl ether (PEGDE) in an alkaline medium. After chemical copolymerisation, reaction with cross-linking agent is stopped. Obtained reaction mixture is neutralised to pH 7.0, then subjected to tangential filtration using a membrane with cut-off threshold 0.22 mcm, concentrated and stirred until complete homogenisation. Starting material used is animal-derived sodium hyaluronate of pharmacopoeial purity, subjected to tangential ultrafiltration to remove low-molecular fragments capable of triggering inflammatory response.
EFFECT: biocompatibility, biodegradability and non-immunogenicity of modified compositions based on sodium hyaluronate allows use thereof in various fields of medicine and veterinary medicine, including surgery and arthroplasty, orthopedics, traumatology, ophthalmology, dermatology, aesthetic medicine and cosmetology.
1 cl, 4 dwg, 10 ex
