FIELD: biotechnology.
SUBSTANCE: present invention relates to the field of biotechnology and medicine, including endoprosthetics, namely, to a method for producing a biopolymer based on sodium hyaluronate and to an application thereof in various fields of medicine. The proposed method for producing a biopolymer based on a modified copolymer of sodium hyaluronate includes creation of cross-linking covalent bonds between the residues of D-glucuronic acid and DN-acetylglucosamine comprised in sodium hyaluronate by introducing two or more cross-linking agents of different molecular weights in a sodium hydroxide solution, wherein each cross-linking agent is independently characterised by a molecular weight within the range from 500 to 8000 Da, wherein the molar ratio of sodium hyaluronate and crosslinking agents is 1:200 to 1:600, wherein each of the agents constitutes (polypropylene glycol)diglycidyl ether. The stage of creating cross-linking covalent bonds is then stopped and the free epoxy groups are bound by means of adding glycine. Also proposed is a biopolymer produced by the described method for use as an endoprosthesis of synovial fluid of a specific structural formula.
EFFECT: invention allows to produce a biopolymer based on a modified sodium hyaluronate copolymer, ensuring most complete replacement of endoprosthetic biological fluid while maintaining the basic natural properties, including structural and transport functions, additionally characterised by low toxicity.
12 cl, 13 dwg, 3 tbl, 10 ex
