FIELD: biotechnology.
SUBSTANCE: disclosed is method of terrilytin producing. Aspergillus terricola H-20 cultivation is carried out. Produced culture liquid is clarified and enzyme is separated. At that, at first stage tangential ultrafiltration culture fluid through membranes with molecular weight cutoff threshold of 100-300 kDa is performed. Second stage involves tangential ultrafiltration with molecular weight cutoff threshold of 2-5 kDa.
EFFECT: method enables to obtain highly pure terrilytin with output of not less than 85 %.
1 cl, 1 dwg, 4 ex
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