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2 590 588

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IPC

A61K39/00(2006-01-01)

C12N15/31(2006-01-01)

C12N15/63(2006-01-01)

C12N15/85(2006-01-01)

C12N15/12(2006-01-01)

(21) (22)

Application

2015131308/10, 2015-06-19

(24)

Start date

2015-06-19

(22)

Actual filing date

2015-06-19

(45)

Published

2016-07-10

(72)

Inventor

Shmarov Maksim MikhajlovichKalugina Irina AlekseevnaSamorukova Aleksandra VladimirovnaAlferova Natalya SergeevnaAtaullakhanov Rustam RavshanovichEremina Natalya VakhitovnaKazej Vasilij Igorevich

(73)

Holder

Obshshestvo S Ogranichennoj Otvetstvennostyu Labs" Innovatsionnyj Tsentr Skolkovo

METHOD FOR PRODUCTION OF PREPARATION BASED ON NON-REPLICATING VIRAL VECTOR EXPRESSING GENE OF HUMAN TOLL-LIKE RECEPTOR AND GENE OF MODIFIED PROTEIN FLAGELLIN Russian patent published in 2016 - IPC A61K39/00 C12N15/31 C12N15/63 C12N15/85 C12N15/12

Abstract RU 2590588 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and concerns a method for production of "Mobilan (M-VM3)" preparation. During updating developed following stable parameters of the production process: batch volume is 1,500 flasks, time of production is 18-22 days, total consumption of the nutrient medium is 44 l, the volume of virus-containing suspension of M-VM3 is 25 l, density of cells during infection in range from 0.8×10⁶ to 1×10⁶ cl/ml; time for gathering cells is on third day (approximately 72 h); temperature during infection is 36 °C, multiplicity of infection 5-10 PFU/cl, total concentration of viral construct at collection is 8×10⁸ PFU/ml.

EFFECT: invention optimises production, increase its profitability, making optimum ratio of production time/amount of produced/amount spent on production.

1 cl, 2 tbl

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RU 2 590 588 C1

Authors

Shmarov Maksim Mikhajlovich

Kalugina Irina Alekseevna

Samorukova Aleksandra Vladimirovna

Alferova Natalya Sergeevna

Ataullakhanov Rustam Ravshanovich

Eremina Natalya Vakhitovna

Kazej Vasilij Igorevich

Dates

2016-07-10—Published

2015-06-19—Filed