METHOD FOR PURIFYING MAMMALIAN AND HUMAN RECOMBINANT ADENOVIRUSES Russian patent published in 2012 - IPC C12N7/02 C12R1/93 

FIELD: medicine.

SUBSTANCE: method involves collection of adenoviruses in a permissive cell culture of line HEK-293, collection of these cells, release of recombinant viruses due to cell destruction by re-frosting, and purification. It involves the use of the recombinant capside-modified adenovirus with modified capside protein pIX carrying a polysaccharide-binding domain specified in a group consisting of a cellulose-binding domain, a dextran-binding domain. The recombinant capside-modified adenoviruses are implanted on the polysaccharide carrier specified from a group consisting of a cellulose carrier for binding with the cellulose-binding domain, the dextran carrier - for binding with the dextran-binding domain. The mixture is incubated. It is followed by the process of purification; for this purpose the polysaccharide carrier with the adenoviruses is settled by centrifugation, while the prepared supernatant is removed. The settled polysaccharide carrier with the adenoviruses is washed in washing buffers, then the adenoviruses are eluted from the polysaccharide carrier. The prepared suspension is separated by centrifugation. The virus-containing supernatant is selected and stabilised.

EFFECT: invention provides preparing the biologically active, chromatographically pure and concentrated adenovirus preparation and reducing a purification time.

15 cl, 4 dwg, 2 tbl, 4 ex