FIELD: bioengineering.
SUBSTANCE: claimed invention discloses a gene expression cassette. The latter contains the DNA-construct where the promoter, the gene, that should be expressed, and the sequence of poly A addition are bonded in such order. Besides, it contains the enhancer(s) or enhancer(s) with UAS doped with its part upstream of its transcription. The latter include at least one enhancer hTERT wherein the said enhancers with UAS are doped directly downstream of transcription from the sequence of poly A addition. The gene expression cassette is used in the procedures of gene expression and protein production coded by this gene as well as in preparations for the detection or treatment of diseases.
EFFECT: increased expression of protein from the gene whereat the enhancer is added upstream of transcription from the promoter.
18 cl, 75 dwg, 10 ex
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