FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, genetic and protein engineering. Invention discloses a recombinant polypeptide, having specific immunogenic properties of isoforms Oct-1, comprising an N-end section of human Oct-1A, a linker and 6 histidine residues; as well as a method for preparing polyclonal antibodies against isoforms of Oct-1 using said polypeptide. Polypeptide has sequence MADGGAASQDESSAAAAAAADSRMNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHGAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQAFHHHHHH.
EFFECT: present invention enables to produce antibodies binding isoforms of human Oct-1A, Oct-1L and Oct-1B and can also be used to produce antibodies with high affinity and specificity with other isoforms of Oct-1.
2 cl, 6 dwg
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